Figure 7.

Paeonol promotes the colocalization of HMGB1 and P65. A, Pathway analysis of the transcriptome is shown. B, The binding rates among HMGB1/P65/P50 were analysed by using mammalian cell hybrid technology. C, HMGB1 was stained red, P65 was stained green, and nuclei were stained blue. P65 was found in the cytoplasm in the control group. In the LPS group, P65 was found in the nucleus, and HMGB1 was found in the cytoplasm. The complex of P65 and HMGB1 was identified in the PL, PM and PH groups. The bar graphs are representative of three independent experiments. *P < .05, ** P < .01 and *** P < .001