FIGURE 6.
BC cell‐intrinsic PD‐L1 promotes autophagy. (A,B) Western blot showing LC3‐II/LC3‐I ratio in control and PD‐L1KO MB49 (A) and RT4 (B) cells. Immunofluorescence staining and quantification(C‐F) and western blot (G‐J) of LC3 puncta at baseline and in response to starvation conditions (EBSS) or pharmacologic autophagy manipulation (chloroquine and 3‐methyladenine). Starvation conditions and treatments are a 6‐hour incubation in either EBSS medium, 20 μM chloroquine (CQ), or 10 mM 3‐methyladenine (3‐MA). Viability assessment (K, L) in MB49 cells 14 h after shown treatments by flow cytometry for Annexin V and propidium iodide (PI). Veh, vehicle. P, (A,B,D,G,K) unpaired t‐test