FIGURE 3.

Scoparone inhibits Ang II infusion‐induced oxidative stress of hearts in vivo. A, 4‐HNE immunohistochemical staining of left ventricular (LV) (n = 4). B, MDA level of hearts, results are expressed as nmol/mg of protein (n = 7‐8). C, measure of RAC1‐GTP, total RAC1, NOX2 and NOX4 in hearts with Western blot (n = 3). D, Rac1‐GTP immunofluorescence staining in cardiomyocytes (CMs, α‐actinin positive) in vivo (n = 4). E, Rac1‐GTP immunofluorescence staining in cardiac fibroblasts (CFs, vimentin positive) in vivo (n = 4).*P < 0.05 vs control group; ^# P < 0.05 vs Ang II group