FIGURE 5.

Scoparone inhibits Ang II‐induced cardiomyocyte hypertrophy by suppressing Rac1 mediated oxidative stress. NRCMs were treated with Ang II (0.1 μmol/L), scoparone (SCO, 50 μmol/L), control or RAC1 overexpression adenovirus (10 moi) for 48 h. A, ³H‐leucine incorporation assay (n = 4). B, Measure of cardiomyocytes surface area with immunofluorescence staining of cardiac troponin I (CTNI) (n = 8). C, Real‐time PCR analysis of ANP, BNP and β‐myosin heavy chain (β‐ MHC) mRNA level (n = 3).*P < 0.05; ^# P < 0.05 vs Ang II group; ^† P < 0.05 vs Ang II + SCO group