FIGURE 2.

Effects of BPP on the expression levels of Collagen I, Collagen III, Collagen IV, Fibronectin and α‐SMA in the renal tissue of unilateral ureteral obstructed(UUO) mice. The mRNA expression level of Collagen I(A), Collagen III (B) and Collagen IV(C) were shown. (D) and (E) Representative results of western blot and quantitative analysis with antibodies against Collagen I, Collagen III and Collagen IV. β‐actin was used as the internal control for normalization of protein loading. (F) Fibronectin and α‐SMA protein expression levels in the renal tissue of UUO mice were detected by immunohistochemistry (400 ×). (G) The quantitative analysis for protein expression levels (positive areas) of fibronectin and α‐SMA in the kidneys. (H) The relative mRNA expression levels (folds) of Fibronectin and α‐SMA in the kidneys. All the experiments were performed in triplicate. Data in (A‐C), (E),(G) and (H) are expressed as mean ± SD, *P < .05 compared with the sham group, ^# P < .05 compared with the UUO group, n = 10. BPP‐L, low dose of BPP (150mg/kg.d); BPP‐H, high dose of BPP(450mg/kg.d); CPN, cyclopamine