FIGURE 3.

The effect of BRG1‐KO on the tyrosine phosphorylation of STAT3 in GBM cells. BRG1 was knocked out in MT330 (A) and LN229 (B) cells by CRISPR‐Cas9 gene editing with three different gRNAs and cell pools were isolated after puromycin selection. As a control, cells were transduced with empty lentiviral vector (EV). Cell lysates were analysed by immunoblotting for BRG1, pY705‐STAT3, pY701‐STAT1, total STAT1 and STAT3, and actin. As a positive control for STAT1 and STAT3 tyrosine phosphorylation, cell lysates were prepared from EV cells that were treated with IFN‐con1 for 30 min