Table 2.
Summary of key metrics of each concentration method. A detailed breakdown of costs and list of equipment used can be found in S14-23. The coefficient of variation (%CV) was determined by calculating the coefficient of variation of the replicates for a single wastewater treatment plant and then averaging that coefficient of variation across all wastewater treatment plants for a given concentration method.
| Method | Startup cost ($) | Consumables cost ($/ sample) | Throughput (hr/6 samples) | LoQ (gc/L WW) | Effective LoQ (gc/L WW) | Average BCoV Recovery Percent | BCoV %CV | pMMoV %CV |
|---|---|---|---|---|---|---|---|---|
| Direct Extraction | $5,650 | $0.14 | 0.1 | 2.56E+05 | 8.39E+06 | 3.84% | 14.6 | 29.9 |
| HA Filtration + Bead Beating | $15,368 | $1.50 | 0.7 | 3.07E+03 | 2.76E+05 | 0.96% | 27.3 | 25.9 |
| HA Filtration + Elution | $11,160 | $5.80 | 0.5 | 2.56E+04 | 3.89E+06 | 0.57% | 20.9 | 31.8 |
| PEG | $20,288 | $11.02 | 4.6 | 2.56E+03 | 2.70E+06 | 0.08% | 39 | 49.8 |
| Ultrafiltration | $9,000 | $12.10 | 1.5 | 7.67E+03 | 2.63E+06 | 0.36% | 24.4 | 49.5 |