Fig. 2.

Schistosoma mansoni venom allergen-like gene Smval6-mediated RNA interference (RNAi) of adult worms depletes both transcript and protein levels. (A) Seven week old adult male and female schistosomes were electroporated with 5 µg of short interfering RNA (siRNA) duplexes targeting either luciferase (siLuc) or Smval6 (siSmval6). After 48 h, total RNA was harvested and subjected to quantitative reverse transcription (qRT)-PCR. Percent knockdown (KD) is indicated. All siRNA and qRT-PCR DNA sequences are included in Supplementary Table S1. (B) After 7 days, total protein was harvested and subjected to SDS-PAGE and western blot analyses using a murine polyclonal anti-rSmVAL6 antisera (Rofatto et al., 2012).