Skip to main content
. 2021 Feb 8;40(6):e106449. doi: 10.15252/embj.2020106449

Figure 6. Rotated Saccharomyces cerevisiae ribosome partially bound by disordered eEF3 ligand.

Figure 6

  • A
    Cryo‐EM reconstruction of the eEF2 bound 80S ribosome adopting a rotated POST‐1 state bearing chimeric ap/P‐ and pe/E‐tRNA as well as a disordered eEF3.
  • B
    Subunit rotation and head swivel observed in the S. cerevisiae eEF3‐80S complex derived from the selected class from the 3D classification shown in (A). 18S rRNA structures illustrating the degree of rotation of the small subunit relative to the large subunit based alignments of the large subunit from a non‐rotated structure reference (PDB ID: 6SNT) (Matsuo et al, 2020). The degree of head swiveling (right side) is illustrated based on alignments of the 18S rRNA body from a non‐headed swivel reference structure (PDB ID: 6SNT) (Matsuo et al, 2020).
  • C, D
    80S ribosome in rotated (C) PRE‐3 state containing an A/A‐ and P/E‐tRNA and (D) PRE‐4 state bearing the hybrid A/P‐ and P/E‐tRNA and bound by the disordered eEF3 ligand.
  • E, F
    Representation of the rotation of the subunit and the 40S‐head swivel shown for volumes depicted in (C) and (D), respectively.