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. 2021 Feb 15;40(6):e105543. doi: 10.15252/embj.2020105543

Figure EV3. Confirmation of bone marrow transplant radiation chimaerism.

Figure EV3

  1. Strategy for making bone marrow chimaeras.
  2. Chimaerism was confirmed 12 weeks post‐transplant in spleen cells by flow cytometric analysis of congenic markers on leucocytes (CD45.1, CD45.2). Flow plot shows representative plot from one C57BL/6 WT (CD45.1) bone marrow → δWD (CD45.2) recipient chimaera and one C57BL/6 WT (CD45.1) bone marrow → littermate control (CD45.2) recipient chimaera. All animals were > 95% chimaeric.