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. 2020 Dec 13;13(2):583–591. doi: 10.1111/os.12886

Fig 4.

Fig 4

P21 and P53 regulated the proliferation and senescence of nucleus pulposus cell (NPC) senescent NPC‐derived exosomes (SNPC‐Exo) treatment. (A, B) The expression of P21 and P53 in NPC was inhibited by siRNA transfection. (C, D) Relative percentage of SA‐β‐gal positive cells among groups after corresponding treatments. (E) Relative fold change of absorbance among groups after Corresponding treatments at 450 nm. (F) The cell cycle phases of NPC were determined by flow cytometry analyses. CON, NPC cultured alone; EXO, NPC treated with SNPC‐Exo; EXO+siP21, NPC treated with SNPC‐Exo and P21 siRNA; EXO+siP53, NPC treated with SNPC‐Exo and P53 siRNA; NC, NPC treated with siRNA negative control; NS, no significance; siRNA, NPC treated with P21 or P53; siRNAEXO+NC, NPC treated with SNPC‐Exo and siRNA negative control. All data are shown as mean ± SD. *P < 0.05. **P < 0.01. ***P < 0.001.