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. 2020 Dec 7;40(6):e105496. doi: 10.15252/embj.2020105496

Figure 5. Loss of m5C impacts translation efficiency of leucine and proline codons.

Figure 5

  1. Fraction of polysomal ribosomes quantified from polysome profiles in the wild‐type and noNSUN strains subject to a 4 h heat shock at 27°C. ns = non‐significant.
  2. Heat map showing P‐site codon occupancy according to the colour scale at 20 and 27°C. Red and blue refer to enhanced and reduced codon occupancy, respectively, in the noNSUN strain relative to wild type. Leucine and proline codons are marked in red.
  3. Ribosome‐protected fragment (RPF) counts in each sample plotted along ife‐1 and pat‐10 CDS. Vertical grey lines indicate UUG codons.
  4. Translation efficiency of UUG‐enriched, leucine‐enriched, proline‐enriched and random genes in each sample. A gene was considered enriched in a certain codon when the proportion of this codon in the gene was at least 3‐fold higher than the proportion of the same codon across the transcriptome.

Data information: In (A–E), n = 3 biological replicates. In (A), data are presented as mean ± SEM, unpaired two‐tailed Student’s t‐test. In (D), box plots show the median (central band) and IQR (boxes) ± 1.5 × IQR (whiskers), Welch’s t‐test, P‐value < 0.05.