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Co-culture of astrocytes and neurons. Neurons were cultured separately in 24-well plates for at least 7 days. Astrocytes were seeded separately on the Transwell® permeable inserts. Once they reached 80% confluency, the inserts with astrocytes were moved to the wells with cultured neurons. After treating of astrocytes with treatment compounds, the cells were co-cultured for 24 h.
