Table 2.

The affinity parameters for RHA0385 and its variants complexed to vHA (A/chicken/Kurgan/3654at/2005 (H5N1)) and rHA (A/Vietnam/1203/2004 (H5N1)). The values of rate constants of association (k_on, 1 × 10⁵ M⁻¹ × s⁻¹) and dissociation (k_off, 1 × 10⁻³ s⁻¹), and apparent dissociation constant (aK_D, nM) are given.

	I Strategy, rHA			II Strategy, vHA
	kon, 1 × 105 M−1 × s−1	koff, 1 × 10−4 s−1	aKD, nM	kon, 1 × 105 M−1 × s−1	koff, 1 × 10−4 s−1	aKD, nM
RHA0385	2.6 ± 0.2	1.5 ± 0.7	0.6 ± 0.3	41 ± 6	2.0 ± 0.6	0.05 ± 0.02
G7nt	3.5 ± 0.2	3 ± 1	0.7 ± 0.3	19 ± 5	2.6 ± 0.6	0.13 ± 0.07
G7n3t	7.4 ± 0.6	3 ± 1	0.4 ± 0.2	28 ± 5	4 ± 1	0.16 ± 0.07
G7n5t	5.5 ± 0.2	1.6 ± 0. 8	0.3 ± 0.2	33 ± 6	1.5 ± 0.5	0.04 ± 0.02
G7nt-T_T_T	1.04 ± 0.04	0.8 ± 0.2	0.7 ± 0.2	8.0 ± 0.8	5 ± 2	0.6 ± 0.3
G7nt-A_T_T	0.65 ± 0.08	0.29 ± 0.09	0.4 ± 0.2	21 ± 4	4 ± 1	0.2 ± 0.1
G7nt-T_A_T	1.80 ± 0.05	1.1 ± 0.4	0.6 ± 0.2	37 ± 9	2.6 ± 0.7	0.07 ± 0.04
G7nt-T_T_A	0.65 ± 0.01	0.5 ± 0.1	0.8 ± 0.2	10 ± 2	7 ± 2	0.7 ± 0.3
G7nt-T_A_C	-	-	-	16 ± 4	5 ± 1	0.3 ± 0.1
G7nt-A_A_C	-	-	-	15 ± 3	4 ± 1	0.3 ± 0.1
G7nt-A_A_A	0.75 ± 0.09	0.4 ± 0.2	0.6 ± 0.4	22 ± 3	5 ± 2	0.2 ± 0.1
G7-TTATTAA_A_C	3.10 ± 0.09	1.2 ± 0.6	0.4 ± 0.2	33 ± 6	3 ± 1	0.09 ± 0.05
G7-TTA_A_C	2.0 ± 0.2	0.7 ± 0.4	0.4 ± 0.2	54 ± 7	2.0 ± 0.3	0.04 ± 0.01