Table 4.

Relationships between structural and affinity parameters of RHA0385 variants in terms of coefficient of determination (R²) values and p-values for F-statistics of linear regression (given as follows: R²/p-value. These values are given for the dependencies of kinetic constants of association (k_on) and dissociation (k_off), or Gibbs energy of binding (ΔG_b,303) for aptamer complexes with rHA and vHA versus the different structural parameters of aptamers. The strongest correlations with R² ≥ 0.6 and p-value ≤ 0.005 are highlighted.

	I strategy, rHA			II strategy, vHA
	kon, 1 × 105 M−1 × s−1	koff, 1 × 10−4 s−1	ΔGb,303, kJ/mol	kon, 1 × 105 M−1 × s−1	koff, 1 × 10−4 s−1	ΔGb,303, kJ/mol
Major loop length, nt	0.64/0.003	0.60/0.005	0.22/0.2	0.18/0.1	0.39/0.02	0.35/0.03
Monomer content, %	0.45/0.02	0.27/0.1	0.23/0.1	0.15/0.2	0.08/0.3	0.11/0.3