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. 2021 Feb 28;22(5):2451. doi: 10.3390/ijms22052451

Figure 4.

Figure 4

Expression of osteoclast marker genes in PBMC derived osteoclasts after 14 days of differentiation. Fold changes were calculated by the ΔΔCT method and related to the samples without Cu2+. Samples of three different PBMC donors were used (each n = 3, n = 9 in total per group), mean ± upper/lower limit. Statistical differences were calculated from DCT values. ** p < 0.01.