Table 2.
List of antibodies used in this study.
| Antibody | Target | Cellular Location | Dilution | Host Species | Class | Manufacturer | Antibody ID |
|---|---|---|---|---|---|---|---|
| MBP | Myelin | Plasma Membrane |
1:500 | Rat | Monoclonal clone 12 |
Biorad | aa 82–87 |
| NFH | Neurons/ Axons | Cell Body | 1:1000 | Rabbit | Polyclonal | Abcam | Ab 8135 |
| Iba-1 | Microglia | Plasma membrane | 1:500 | Guinea pig | Polyclonal | Synaptic Systems | 234 004 |
| PDGFRα | OPCs | Plasm membrane |
1:200 | Goat | Polyclonal | RD Systems | AF 1062 |
| CC1 | Mature oligodendrocytes |
Cell body | 1:200 | Mouse | Monoclonal clone CC1 |
Merck Millipore |
OP 80 |
| Olig2 | Oligodendrocyte lineage |
nucleus | 1:200 | Rabbit | Polyclonal | Merck Millipore |
AB 9610 |
Abbreviations: MBP = myelin binding protein; NFH = neurofilament heavy polypeptide; PDGFRα = platelet-derived growth factor receptor α; OPCs=oligodendrocyte precursor cells.