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. 2021 Feb 28;13(5):1011. doi: 10.3390/cancers13051011

Figure 2.

Figure 2

KAN0438757 effects on PFKFB3 expression in cancer cells and HUVECs. (A) KAN0438757 synthetization protocol steps. (B) Immunoblot. PFKFB3 inhibition in colorectal cancer cells and HUVECs upon 12 h treatment with KAN0438757. (C) Immunoblot relative quantification by densitometry (relative to B-ACTIN loading control). (D) RT-PCR analysis. Relative mRNA expression of PFKFB3 in HCT-116, HT-29 and HUVEC cells upon 4, 12, 24 and 48 h treatment with KAN0438757. Data is ± SEM. * p < 0.05, *** p < 0.001, **** p < 0.0001.