Figure 4.

PFKFB3 inhibition by KAN0438757 reduces cancer cell proliferation and induces cell death in a concentration-dependent manner. (A–D) xCELLigence assay performed with HCT-116 and HT-29 colorectal cancer cells, HUVECs and normal colon epithelial cells during 96 h under different concentrations of KAN0438757 (continuous line); the dotted lines represent the fitted saturation curve in logarithm. (E) CellTiterBlue assay performed with HCT-116, HT-29, HUVECs and normal colon epithelial cells during 48 h. (F) LDH assay performed with HCT-116, HT-29, HUVECs and normal colon epithelial during 48 h. Data is $\pm$ SEM. (A–F) Combined data from three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.