Figure 1.

ANT1 does not contribute to the basal proton leak. Total membrane conductance (G_m) was measured at different membrane potentials (ΔΦ) and membrane compositions (s. legend). Planar bilayer membranes were made of 45:45:10 mol % PC:PE:CL. Lipid concentration was 1.5 mg/(mL of buffer solution). Protein concentration measured by BCA assay was 4 µg/(mg of lipid). Buffer contained 50 mM Na₂SO₄, 10 mM Tris, 10 mM MES and 0.6 mM EGTA at pH = 7.34 and T = 306 K. ADP, ATP and CATR were added at concentrations 2 mM, 2 mM and 100 µM. Lines represent the least square regression fit of an exponential function to the data. Data are the mean ± SD of at least three independent experiments.