Fig. 2.

Sucrose promotes superoxide production and ROS-regulated transcripts in dark-adapted seedlings. Transcript levels of representative ROS-associated genes identified as sugar-regulated from RNA-Seq that are (A) from the GO class “responsive to oxygen-containing compound” or (B) identified from a previous study (19) (means ± SD, n = 3). (C) Histochemical stains for hydrogen peroxide (DAB) and superoxide (NBT) in 10-d-old, dark-adapted Col-0 seedlings treated with 30 mM mannitol or sucrose. (D) DAB and NBT stain intensity in seedlings grown as in C (means ± SD, n = 6; *P < 0.05 from mannitol; Bonferroni-corrected t test). (E) L-012 luminescence in dark-adapted Col-0 treated with 30 mM mannitol or sucrose (means ± SEM, n = 6).