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. 2021 Mar 4;118(10):e2020646118. doi: 10.1073/pnas.2020646118

Fig. 3.

Fig. 3.

Modifiers of superoxide inhibit responses to sucrose. (A) Luciferase luminescence in dark-adapted CCR2p:LUC seedlings treated with 30 mM mannitol or sucrose in the presence of DMSO, 10 µM DPI, 2 µM MV, or 200 µM 3-AT (means ± SEM, n = 6). (B) L-012 luminescence in dark-adapted Col-0 treated as in A (means ± SEM, n = 6). (C) Histochemical NBT stain for O2– and DAB stains for H2O2 in dark-adapted Col-0 seedlings treated with 30 mM mannitol or sucrose in the presence of 0.1% DMSO or 10 µM DPI. (D) Stain intensity in Col-0 seedlings 4 h (NBT) or 0.5 h (DAB) after treatment as in A (n = 6; *P < 0.05; t test). (E) Inhibition of response of luciferase luminescence to 30 mM sucrose in dark-adapted CCR2p:LUC seedlings in the presence of 0 (0.1% DMSO), 1, 5, or 25 µM DPI. (means ± SEM, n = 3; *P < 0.05 from DMSO; Bonferroni-corrected t test). (F) Transcript level of CCR2 and WRKY60 relative to UBQ10 in dark-adapted Col-0 seedlings 8 h after treatment with 30 mM mannitol, sucrose, or sucrose with 10 µM DPI (means ± SD, n = 4; *P < 0.05 from mannitol; Bonferroni-corrected t test. (G) Hypocotyl length and root length of 5-d-old dark-grown Col-0 seedlings grown on 1/2 MS with or without 30 mM mannitol or sucrose, 0.1% DMSO, or 1 µM DPI (means ± SD, n = 10; *P < 0.05 from 1/2 MS; Bonferroni-corrected t test).