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. 2021 Mar 4;118(10):e2020646118. doi: 10.1073/pnas.2020646118

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Fig. 4. — Modifiers of superoxide affect modulation of circadian rhythms by sucrose. (A) Normalized luciferase luminescence in TOC1p:LUC seedlings in continuous low light with 30 mM mannitol (blue) or sucrose (red) in the presence of 0.1% DMSO or 10 µM DPI, 2 µM MV, or 200 µM 3-AT (means ± SD, n = 4). (B) Circadian period estimates of luciferase luminescence in TOC1p:LUC seedlings in A (means ± SD, n = 4; *P < 0.05 from mannitol; Bonferroni-corrected t test). (C) Luciferase luminescence in TOC1p:LUC, PRR7p:LUC, and CCA1p:LUC seedlings for 24 h in light/dark treated as in A (means ± SD, n = 4). (D) Phase of rhythmic O₂^–- and H₂O₂-responsive transcripts in continuous light. Values are enrichment (observed/expected) of up- and down-regulated genes in each 4-h phase window (*P < 0.01; χ²).