Table 2.
Thermodynamic and kinetic parameters for boronic acid oxygen exchange and binding to saccharides and Nopoldiol–PEG
| Saccharide binding, Ka (M−1)* | Nopoldiol–PEG binding | |||||
| Boronic acid | d-fructose | d-glucose | Neu5Ac | Ka (M−1)† | kon (M−1 · s−1)‡ | koff (s−1)§ |
| PBA | 128 ± 20 | 5 ± 1 | 13 ± 1 | 3,000 ± 1,600 | 3.4 ± 1.8 | (1.1 ± 0.9) × 10−3 |
| BL | 336 ± 43 | 28 ± 4 | 43 ± 5 | 550 ± 20 | >50 | >(9.1 ± 0.3) × 10−2 |
| BOL | 23 ± 2 | 1.7 ± 0.1 | 6.0 ± 0.5 | 2,300 ± 380 | 0.12 ± 0.05 | (5.2 ± 2.3) × 10−5 |
*
Values for BOL are the mean ± SD of at least five independent measurements (SI Appendix, Fig. S8). Values for PBA and BL are from ref. 65.
†
Mean ± SD of at least four independent measurements.
‡
Values are the mean ± SD of three independent measurements (SI Appendix, Figs. S9 and S10). The value of kon for BL was too large to be determined with 1H-NMR spectroscopy.
§
Calculated as koff = kon/Ka.