Fig. 1.

SNX1 is sufficient for membrane binding and tabulation. (A) Domain organization of SNX1 (NP_062701.2). PX, Phox homology domain; BAR domain. (B) Binding of SNX1 to liposomes of varying sizes (as indicated) is assessed by coprecipitation assay. Supernatant, S; Pellet, P. (C) Negative-stain EM visualizing liposomes incubated either with (first row) or without (second row) SNX1 (scale bar, 200 nm). The diameters of the liposomes are also indicated. (D) Statistical histogram of the diameters of tubules generated by SNX1 is plotted as a percentage of all tubules; n = 41 for 50 nm liposomes, n = 62 for 100 nm liposomes, n = 60 for 200 nm liposomes, n = 61 for 400 nm liposomes, and n = 70 for 1,000 nm.