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. 2021 Mar 3;118(10):e2007724118. doi: 10.1073/pnas.2007724118

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Copyright © 2021 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 2. — CCK_E neurons of the ICC have large synaptic terminals in the vMGB. (A) A mixture of two AAVs was stereotaxically injected into the ICC to express EGFP in CCK_E neurons. EGFP intensity was enhanced with a biotinylated antibody and visualized with Ni-DAB stain. Gray planes of section denote corresponding locations in B and C. (B) Unilateral ICC injection site (dotted circle) shown in a 65-μm horizontal section. (C) A 65-μm horizontal section from the injection in B. Axons from CCK_E neurons travel through the brachium of the IC toward the ventral division of the MGB (vMGB). Groups of terminals cluster in the vMGB. (D) Representative micrographs demonstrating the diversity of terminal morphology in the vMGB from the injection in B. (E and F) Terminal boutons from CCK_E neurons in the vMGB were reconstructed. Histograms show the distribution of bouton diameter and bouton area (n = 1,079 boutons, n = 2 gerbils).