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. 2021 Mar 2;26(5):1324. doi: 10.3390/molecules26051324

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Scatter plots of the standard deviations (SD) of measurement results as plotted against the combined nominal concentrations of (A) ATR + ATRL and (B) ATR + MET. ATR, ATRL, 2OATR, 2OATRL, 4OATR and 4OATRL were spiked to 20 independent blank serum samples at 20 spiking levels (detailed in Supplementary Table S2). Each data point shows the SD of the combined concentrations. The fitted lines and curves have been obtained by applying various linear and nonlinear regression algorithms to the data points. Black, unweighted linear least squares regression. Blue, weighted linear least squares regression with 1/concentration² weights. Green, Theil’s regression. Red, 2nd-order unweighted nonlinear least squares regression. Orange, 3rd-order unweighted nonlinear least squares regression.