Table 1.

Primers used for indel detection

sgRNA target site	Forward primer (5′−3′)	Tm (°C)	Reverse primer (5′−3′)	Temperature (°C)	PCR size
GGTA1 exon 7	GCC​AGT​CAC​CAC​AAG​CCA​TG	63.5	TGG​CCC​TGT​GAC​ACC​ATT​CT	63.5	362 bp
pB2M exon 1	CCA​CCC​AGT​CCA​ACC​TTT​GCC	65.5	CCA​GAG​TTA​GCG​CCC​GGA​GT	65.8	377 bp
MYBPC3 Δ6 to 23	CTT​GAG​AGG​CTG​TTG​TTG​CTG​G	66.7	GCA​CAC​CCC​ATC​TCT​GAT​TCC​T	67.2	1,225 bp
EGFP	ATG​GTG​AGC​AAG​GGC​GAG​GAG​CT	70.0	CGT​CCT​CGA​TGT​TGT​GGC​GGA​TC	66.9	523 bp
chB2M	CAA​GGT​GCA​GGT​GTA​CTC​C	59.4	ACT​TGT​AGA​CCT​GCG​GCT​C	61.1	269 bp