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Apir mutants tolerate the endogenous expression of Api. (A and B) Spot test for WT or Apir mutant E. coli cells transformed with the pApi(UAG) plasmid. BL21 Apir cells contained a mutant (R81C) uL16 ribosomal protein encoded in the rplP gene (1) (A); SQ110 Apir mutants contained the C2666U mutation in the 23S rRNA gene rrlE or the R277L mutation in RF1 encoded in the prfA gene (B).
