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. 2021 Mar 1;118(10):e2020922118. doi: 10.1073/pnas.2020922118

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Fig. 5. — Oxygen supply to the symbiotic organ via the tracheal network is important for gut symbiosis. Chitin staining (first row), 3D reconstruction of the DTN (second row), colonization by Burkholderia (third row), and crypt morphology (fourth row) in the M4 of Sym (Sym^control), RpBnl-RNAi (Sym^RpBnl-RNAi), RpTrh-RNAi (Sym^RpTrh-RNAi-), and RpSima-RNAi (Sym^RpSima-RNAi) insects. Images were obtained by epifluorescence microscopy in the first and third rows and by laser scanning confocal microscopy in the second and fourth rows. White, chitin; yellow, DTN; green, gut symbiont; blue, host nuclei; red, host cytoskeleton.