Fig. 2.
RSV NS2 coimmunoprecipitates with RIG-I and MDA5. (A) Empty expression plasmid (empty vector), FLAG-full-length RIG-I (FLAG-RIG-IFL) or FLAG- full-length MDA5 (FLAG-MDA5FL) were cotransfected with HA-tagged NS2 expression plasmid in HEK 293T cells in the absence (Top) or presence (Bottom) of poly I:C transfection. (B) Cells were cotransfected with empty vector, HA-RIG-ICARDs, or HA-MDA5CARDs with FLAG-NS2. (C) Cells were cotransfected with empty vector, HA-RIG-ICARDs, HA-MDA5CARDs, HA-RIG-I helicase/CTD (RIG-IΔCARDs), or HA-MDA5 helicase/CTD (MDA5ΔCARDs) with FLAG-NS2. (D) Cells were cotransfected with HA-RIG-ICARDS, HA-MDA5CARDs, or HA-MAVS with empty vector or FLAG-NS2. Western blots of IP and whole-cell lysates (WCL), including β-tubulin, are shown. Shown are representative results from experiments repeated in triplicate.