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. 2021 Mar 2;13(5):1046. doi: 10.3390/cancers13051046

Figure 6.

Figure 6

SiRNA targeting of PVT1 exon 9 induces claudin 4 protein re-expression in MDA MB 231 CL TNBC cells. (A) MDA MB 231 CL TNBC cells were transfected with PVT1 exon 9 specific siRNAs (siPVT1 exon 9) for 24 h. Western blotting was performed using specific antibodies against claudin 1, claudin 3, claudin 4, claudin 7 and E-Cadherin. SiRNA targeting of PVT1 exon 9 induced claudin 4 protein re-expression in MDA MB 231 CL TNBC cells in comparison to MDA MB 231 CL TNBC cells transfected with only control scramble non-targeting siRNA (siScramble). (B) Quantification of relative claudin 4 protein expression normalized to GAPDH protein expression; N = 2.