Figure 6.

Targeting CDCA8 enhances apoptotic signaling through activation of the ATF3 and GADD34 tumor suppressors. (A) Western blot analysis of the expression of CDCA8 and the indicated proteins functionally related to cell proliferation, apoptosis, or cell cycle regulation. The proteins were prepared at 48 h after transfection with CDCA8-1siRNA. β-actin was detected as a loading control. * exact position of target protein band when measured with molecular weight markers (refer to Figures S5 and S6). (B–D) The correlation among the expressions of CDCA8, ATF3, and GADD34 in HCC tissues. TCGA data showed that CDCA8 had a negative correlation with ATF3 (B) and GADD34 (C), and there was a positive association between ATF3 and GADD34 (D).