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Silencing of CDCA8 inactivates AKT/β–catenin signaling and inhibits sphere formation. (A) Western blot analysis of CDCA8 and the indicated proteins functionally involved in AKT/β–catenin signaling. (B) Detection of sphere formation in HCC cells treated with NCsiRNA or CDCA8-1siRNA. * exact position of target protein band when measured with molecular weight markers (refer to Supplementary Figure S7). ** p < 0.01 vs. control. Scale bar = 100 μm.
