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. 2021 Mar 2;13(5):1055. doi: 10.3390/cancers13051055

Figure 9.

Figure 9

CDCA8 silencing suppresses HCC growth in vivo. (A) Kinetics of tumor growth: Huh7 cells transfected with NCsiRNA or CDCA8-1 siRNA for 24 h were subcutaneously injected into nude mice. Tumor diameters were measured on the indicated days with digital calipers (** p < 0.01 (n = 6 vs. n = 6)) by normality assumption test. (B,C) Average tumor weight (B) and gross tumor morphology (C) at 17 days after Huh7 cell transplantation. (D) Detection of mRNA expression of CDCA8, ATF3, and GADD34 genes in tumors 24 h after the third injection of CDCA8-1siRNA. ** p < 0.01; *** p < 0.001 vs. control. (E) Levels of CDCA8 and the indicated proteins in tumors 48 h after the third injection of CDCA8-1siRNA. (F) Detection of proliferative tumor cells and CDCA8 target protein in tumors 1 week after the third injection of CDCA8-1siRNA. Scale bar = 100 μm.