Figure 4.

KIT 10 did not affect COX enzymatic activities. (A) To assess COX-1 activity, cells were treated with different concentrations of KIT 10 (0.1–10 µM) for 15 min before the addition of 15 μM of arachidonic acid (AA). PGE₂ in the supernatants was measured after an additional 15 min. (B) To assess COX-2 activity, cells were stimulated for 24 h with LPS (10 ng/mL) and then treated with different concentrations of KIT 10 for 15 min. After this incubation time, 15 μM of AA were added and PGE₂ in the supernatants was measured by enzyme immune assay. Data are expressed as mean ± SEM of at least three new cultures/group. * p < 0.05 and *** p < 0.001 with respect to control (One-way ANOVA followed by the Newman-Keuls post-test).