Figure 2.

Overexpression of ZIC5 promoted CRC cell proliferation through activating CDC25/CDK1/cyclin B1 signaling. SW620 cells and HCT116 cells were transfected with ZIC5 plasmid and siZIC5 respectively. A – Transfection efficiency was examined by qRT-PCR and western blot (B). C – MTT assay showed that SW620 cells transfected with ZIC5 plasmid had higher proliferation capacity, D – whereas inhibition of ZIC5 decreased HCT116 cell proliferation E – Cell cycle analysis revealed that in SW620 cells with overexpression of ZIC5, a larger proportion of cells remained in the S phase, whereas in HCT116 cells with ZIC5 knockdown, G0/G1 phase cells were increased F, G – Western blot results showed that expression of cell cycle related proteins was up-regulated and CDC25C was translocated into the nucleus and was responsible for activation of downstream cascades (***p < 0.001)