Leptin: |
40 |
Forward |
5’-AGA TCC TCA CCA GTA TGC CTT-3’ |
|
Reverse |
5’-CTC TGT GGA GTA GCC TGA AGC-3’ |
|
Cycle protocol: |
30” at 95°C, 30” at 59°C, 30” at 72°C |
|
Adiponectin: |
40 |
Forward |
5’-GGC ATG ACC AGG AAA CCA C-3’ |
|
Reverse |
5’-TTC ACC GAT GTC TCC CTT AGG-3’ |
|
Cycle protocol: |
15” at 95°C, 30” at 59°C, 30” at 72°C |
|
Resistin: |
40 |
Forward |
5’-ATG AAA GCT CTC TGT CTC CTC-3’ |
|
Reverse |
5’-GCA CTG CGC GCG ACC TCA-3’ |
|
Cycle protocol: |
30” at 95°C, 30” at 56°C, 30” at 72°C |
|
RPLP0: |
40 |
Forward |
5’-CAG ATT GGC TAC CCA ACT GTT-3’ |
|
Reverse |
5’-GGC CAG GAC TCG TTT GTA CC-3’ |
|