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. 2021 Feb 26;17(2):323–336. doi: 10.5114/aoms/92118

Table I.

Primers sequences and cycling conditions

Gene Sequence Cycle number
Leptin: 40
 Forward 5’-AGA TCC TCA CCA GTA TGC CTT-3’
 Reverse 5’-CTC TGT GGA GTA GCC TGA AGC-3’
 Cycle protocol: 30” at 95°C, 30” at 59°C, 30” at 72°C
Adiponectin: 40
 Forward 5’-GGC ATG ACC AGG AAA CCA C-3’
 Reverse 5’-TTC ACC GAT GTC TCC CTT AGG-3’
 Cycle protocol: 15” at 95°C, 30” at 59°C, 30” at 72°C
Resistin: 40
 Forward 5’-ATG AAA GCT CTC TGT CTC CTC-3’
 Reverse 5’-GCA CTG CGC GCG ACC TCA-3’
 Cycle protocol: 30” at 95°C, 30” at 56°C, 30” at 72°C
RPLP0: 40
 Forward 5’-CAG ATT GGC TAC CCA ACT GTT-3’
 Reverse 5’-GGC CAG GAC TCG TTT GTA CC-3’