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. 2021 Mar 3;22(5):2512. doi: 10.3390/ijms22052512

Figure 5.

Figure 5

The content of chlorophyll and photosynthetic proteins. (A) Chlorophyll content of WT and ACM1-RNAi-1 seedlings. Chlorophyll was quantified from 7-day-old seedlings cotyledons and 14-day-old seedlings’ true leaves. Values given are μg/g fresh weight ± SD. The asterisks indicate significant differences (Student’s t-test; *** p < 0.001). The average of three replicates is shown. (B) Immunoblot analysis of photosynthetic proteins. Total proteins from 7-day-old cotyledons and 14-day-old true leaves of WT and ACM1-RNAi-1 were separated by 10% SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis). Probed using specific anti-D1, anti-LHCII, anti-PsaA, anti-PsaC, anti-Cyt f, anti-CF1α, and anti-RbcL. ACTIN is used as a control. The experiments were repeated three times at least with similar results. (C) Proteins immunodetected from (A) were analyzed by ImageJ software. Values (means ± SE; n = 3 independent biological replicates) are given as ratios to protein amounts of the WT and ACM1-RANi-1. *** P < 0.001, by Student’s t-test. T refers to the proteins in true leaves of ACM1-RNAi-1 seedlings.