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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Mol Microbiol. 2011 Jul 6;81(4):1061–1077. doi: 10.1111/j.1365-2958.2011.07753.x

Fig. 1.

Fig. 1.

Germination protein fusions are at least partially functional. Spores were germinated with (A) AGFK and (B) L-alanine and the decrease in the OD600 was monitored. The genotype of the strains used in germination assays is shown in (C), arrows indicate the relative position of promoters as well as the direction of transcription. The symbols for the strains used to generate spores are: ■, PS4150; ○, GerAA-mCherry (KGB174 – Ci); △, GerBA-GFP (KGB202 – Cii); ⁕, GerKA-GFP (KGB203 – Ciii); □, GerKB-GFP (KGB08 – Civ); and ◇, GerD-GFP (KGB52 – Cv). Strains KGB174 and KGB202 contain two copies of their respective fusion genes, and KGB203, KGB08 and KGB52 contain one copy of their respective fusion genes expressed under the control of their native promoters, shown in (C). All these strains lack the full-length wild-type gene as shown in (C). There was no germination when spores were incubated without AGFK or L-alanine. Results similar to those with GerKB-GFP spores were also obtained with spores carrying GerKB-mCherry (KGB34).