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. Author manuscript; available in PMC: 2021 Mar 15.
Published in final edited form as: Mol Cell. 2020 Nov 19;81(1):139–152.e10. doi: 10.1016/j.molcel.2020.10.035

Figure 4. Cryo-EM structure of the FtRNAPs70-(MglA-SspA)-ppGpp-PigR-DNA complex.

Figure 4.

A Cryo-EM structure of the FtRNAPσ70-(MglA-SspA)-ppGpp-PigR-DNA complex. The PigR C-tail is modeled from the crystal structure. Above the structure is the full promoter sequence used in the structure, with promoter elements labeled. B DNA sequences of PigR controlled genes showing just the promoter regions. −35 and −10 elements are indicated, PRE elements are colored brown and AT-rich UP elements are light yellow. C Images of the FtRNAPσ70-(MglA-SspA)-ppGpp-PigR-DNA density map at various contour levels. Right shows a close up of the linker region, which leaves ambiguous which NTD is linked to which CTD.