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. Author manuscript; available in PMC: 2022 Feb 2.
Published in final edited form as: Cell Metab. 2021 Feb 2;33(2):334–349.e6. doi: 10.1016/j.cmet.2021.01.003

Figure 1. Severe mitoribosomal stress in POMC neurons causes maturity-onset obesity and glucose intolerance.

Figure 1.

(A–C) Representative body images, body weights, lean mass, and fat mass in Crif1f/f and Pomc-Cre; Crif1f/f mice at the indicated ages (n = 7–8).

(D, E) Food intakes and energy expenditure measured at the indicated ages (n = 6–9).

(F) Results of glucose tolerance tests (GTT) and insulin tolerance tests (ITT) (n = 5–8).

(G, H) Immunohistochemistry of POMC neurons using β-endorphin (β-END) antibody and hypothalamic Pomc mRNA expression measurement at 5, 14 and 35 weeks of age (n = 6–7). 3V, third ventricle; ARH, hypothalamic arcuate nucleus. Scale bars, 50 μm.

Results are presented as a mean ± SEM. *p < 0.05, and ***p < 0.001 vs. Crif1f/f littermates. NS, not significant. See also Figure S1