(A–C) Body weights, lean mass and fat mass of Crif1f/f and Pomc-Cre; Crif1f/+ mice, determined at the indicated ages (n = 6–8).
(D) Food intakes in the mice at the indicated ages (n = 7–8).
(E, F) Energy expenditure and locomotor activity in Crif1f/f and Pomc-Cre; Crif1f/+ males at 8 weeks of age (n = 6).
(G, H) Rectal temperatures during 4°C cold exposure and skin temperature in young Crif1f/f and Pomc-Cre; Crif1f/+ males (n = 5–8).
(I–K) Comparison of body weights, food intake, and energy expenditure during a high fat diet (HFD, 60% fat) challenge between Pomc-Cre; Crif1f/+ males and Crif1f/f controls (n = 7–8).
Results are presented as a mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Crif1f/f controls. NS, not significant. See also Figure S2