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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Plant J. 2020 Jul 11;104(1):113–123. doi: 10.1111/tpj.14908

Figure 2: Overexpression of COP1-HA and TAP-SPA1 promotes the degradation of HEC2-GFP through the 26S proteasome-mediated pathway in the dark.

Figure 2:

(A and C) Immunoblots showing HEC2-GFP protein accumulation in the HEC2-GFP and HEC2-GFP/COP1-HA (A) or HEC2-GFP and HEC2-GFP/TAP-SPA1 (C) double transgenic lines, respectively, with and without proteasome inhibitor (40 μM Bortezomib (BORTZ), 3hrs) treatment. Seedlings were grown in the dark for 4 days. Total protein was separated on an 8% SDS-PAGE gel, blotted onto PVDF membrane and probed with anti-GFP or anti-RPT5 antibodies. (B and D) Bar graphs show the HEC2-GFP protein levels in seedlings of the corresponding genotypes. Error bars indicate standard deviation (n=3). The letters “a” to “c” indicate statistically significant differences between means of relative protein levels among the genotypes based on one way ANOVA followed by Tukey’s HSD (p<0.05).