Fig 6. Effect of pairwise drug combinations of selected HDAC inhibitors with albendazole (ABZ) on parasite viability.

In vitro anthelmintic activity was determined for individual HDAC inhibitors plus ABZ: (A) entinostat, (C) TH65, and (E) TH92; as well as entinostat in combination with (B) TH65, and (D) TH92. (F) Concentrations used for each compound, corresponding to their respective IC₂₅ values. The compounds were tested alone and in combination at different incubation times, using the Mesocestoides vogae tetrathyridia (TTy) motility assay. Relative motility indices were measured from three independent biological replicates, each one in quadruplicate. Error bars represent the standard deviation. Proportional effect indices were calculated at 6 days of incubation (marked with a box). TTy incubated with the drug vehicle (1% DMSO) represented a negative control.