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. 2021 Mar 15;17(3):e1009401. doi: 10.1371/journal.ppat.1009401

Fig 2. Depletion of Ube2m but not Ube2f attenuates dsDNA-induced innate immune response.

Fig 2

(A) Effects of Ube2m deficiency on the expression of Ifnb, Ifna4 and Cxcl10 after ISD stimulation for indicated hours in BMDM. (B) Effects of Ube2m deficiency on the expression of Ifnb, Ifna4, Cxcl10 and Ifit1 after HSV-1 (MOI = 1) stimulation in BMDM. (C) Effect of Ube2f deficiency on the expression of Ifnb, Ifna4 and Cxcl10 after ISD and HT-DNA stimulation in BMDM. (D) Effects of Ube2m deficiency on the phosphorylation of TBK1/IRF3 and the dimerization of IRF3 after ISD stimulation for the indicated time. (E) Effects of Ube2f deficiency on phosphorylation of TBK1/IRF3 and the dimerization of IRF3 after ISD stimulation for the indicated time. (F) Effects of Ube2m deficiency on the protein level of cGAS and STING. Graphs are presented as means ± SEM, data are representative of three independent experiments, *P <0.05; **P <0.01 (One-way ANOVAs followed by Tukey’s post hoc test).