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. 2021 Mar 15;17(3):e1009401. doi: 10.1371/journal.ppat.1009401

Fig 3. Rnf111 facilitates dsDNA-triggered signaling.

Fig 3

(A) dsDNA stimulation promoted the expression of Rnf111 in MEFs (left) and BMDM (right). (B) Effect of Rnf111 deficiency on the expression of Ifnb, Ifna4 and Cxcl10 after ISD and HT-DNA stimulation for indicated hours in BMDM. (C) Effect of Rnf111 deficiency on the expression of Ifnb, Ifna4 and Cxcl10 after HSV-1 (MOI = 0.5) infection in BMDM. (D) Effects of Rnf111 deficiency on IRF3 dimerization and phosphorylation of TBK1/IRF3 after ISD (left) or cGAMP (right) stimulation for the indicated time. (E) Effect of Rnf111 deficiency on the expression of Ifnb and Cxcl10 after cGAMP stimulation for 3h in BMDM. (F) Rnf111 deficiency BMDM were transfected with 100 ng of empty vector (EV, pcDNA4.0) or plasmids for the expression of wild-type His-Rnf111 or His-Rnf111 C937A. 48h after transfection, BMDM were stimulated with ISD for 6h, the transcription of Ifnb, Ifna4 and Cxcl10 were measured by qRT-PCR. Graphs are presented as means ± SEM, data are representative of three independent experiments, *P <0.05; **P <0.01 (One-way ANOVAs followed by Tukey’s post hoc test).