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. 2021 Mar 15;17(3):e1009401. doi: 10.1371/journal.ppat.1009401

Fig 7. UBE2M-RNF111 mediated neddylation pathway is indispensable for the innate defense against HSV-1 infection.

Fig 7

(A) Mice were injected intravenously with HSV-1 (1.5×107 pfu per mouse) for 12h, tissues from Ube2m cKO or the control group were harvest and the relative expression of Ifnb and Cxcl10 in livers, spleens and lungs were measured by qRT-PCR, respectively (n = 6–10). (B) Mice were injected intravenously with HSV-1 (1.5×107 pfu per mouse) for 12h, the serum was harvested and the concentration IFNβ was analyzed with ELISA (n = 6–8). (C) Mice were injected intravenously with HSV-1 (1.5×107 pfu per mouse) for 72h, lungs were harvested and inflammatory cells infiltration were analyzed by H&E staining (left), scale bars represent 100 μm. The presence of inflammation was scored and counted (right, n = 5). (D) Mice of the Ube2m cKO or the control groups were injected intravenously with HSV-1 (6×107 pfu per mouse), and the survival rates were monitored for 7 days (n = 10). (E) Mice were injected intravenously with HSV-1 (1.5×107 pfu per mouse) for 12h, tissues from Rnf111 cKO or the control group was harvest, and the relative expression of Ifnb and Cxcl10 in livers, spleens and lungs were measured by qRT-PCR (n = 6–10). (F) Mice were injected intravenously with HSV-1 (1.5×107 pfu per mouse) for 12h, the serum was harvested and the concentration IFNβ was measured with ELISA (n = 6–10). (G) Mice were injected intravenously with HSV-1 (1.5×107 pfu per mouse) for 72h, lungs were harvested and inflammatory cells infiltration were analyzed by H&E staining (left), scale bars represent 100 μm. The presence of inflammation was scored and counted (right, n = 5). (H) Mice of the Rnf111 cKO or the control groups were injected intravenously with HSV-1 (6×107 pfu per mouse), and the survival rates were monitored for 7 days (n = 10). Graphs are presented as means ± SEM, data are representative of three independent experiments, *P <0.05; **P <0.01 (Mann-Whitney test for C and G, Mantel-Cox test for D and H, One-way ANOVAs followed by Tukey’s post hoc test for the rest).