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A visible blue line represents a positive detection. Detection of uniplex (no.1 and 2), duplex (no. 3 and 4), and triplex bla genes (no. 5 and 6) from the three bla genes (A). The combination of the uniplex blaSHV product with the duplex blaCTX-M and blaOXA products at 1:4, 2:4, 3:4 and 4:4 (v/v) ratios (B). C+; positive DNA control, C-; no template control.
