Figure 3.

O-glucuronidated NVP and 12-D₃NVP formation in primary human and mouse hepatocytes. Cryopreserved primary human hepatocytes (A) and fresh primary mouse hepatocytes (B) were incubated with 10 μM NVP or 12-D₃NVP for 24 or 48 h. O-glucuronidated metabolites extracted from hepatocyte culture medium were measured using uHPLC-MS (Orbitrap) detection. The peak area of the XICs for the following high resolution ions was observed to assay metabolite formation: 459.1510 ± 5 ppm for O-glucuronidated, undeuterated NVP (O-GlucNVP) and 462.1700 ± 5 ppm for O-glucuronidated, trideuterated NVP (O-GlucD₃NVP). Data are representative of the mean ± standard deviation of three (human) or four (mouse) experimental replicates.